This study was focused on the relationship between workplace layout and occupational safety of employees. The purpose of this study was to examine the relationship between workplace design or layout and occupational safety of employees. A population of 300 workers drawn from 5 stratified and selected firms, representing the largest in term of employees and output in the Calabar Free Trade Zone, (CFTZ) Nigeria, constituted the population of the study. A sample of 169, which was representative of the various cadres of workers, was carved from the population with the aid of Krejcie Morgan table. Data was gathered with questionnaire from 169 respondents and hypotheses tested with Pearson product-moment correlation analysis at 0.05 level of significance and 118 degree of freedom. Findings indicated that most workers were dissatisfied with their workplace physical design, such as;the circulation of gangways/walkways, the articulation and functional relationships between spaces, ventilation and the unavailability of fire-fighting equipment, emergency exit doors and proper constructed gangways. Furthermore, findings also revealed that some of the consequences of unsafe and unhealthy work environment include; stress, industrial accidents and the development of chronic diseases, which affects employees physical and mental fitness, as well as, their occupational safety generally. Thus findings emanating from this study is a clarion call for the overhauling and redesigning of workplaces in the CFTZ and industrial space at large. It is also a wake-up call to government regulatory agencies, such as Federal Ministry of Labour and Productivity and Standard Organization of Nigeria (SON) to ensure strict adherence to workplace standards. It was recommended that organizations should provide work environments that are supportive of occupational safety and which guarantees the health and welfare of their workers.